Samberg was born in Berkeley, California. His mother, Marjorie "Margi" (née Marrow), is an elementary school teacher, and his father, Joe, is a photographer. He has two sisters, Johanna and Darrow. He is Jewish, although he describes himself as "not particularly religious. " His grandfather was industrial psychologist and philanthropist Alfred J. Marrow, and his third cousin is U. S. Senator Tammy Baldwin, of Wisconsin.